Objective To meet the growing demand for specialized professionals in biotechnology production, the biotechnical drug course requires targeted reform in its content and teaching method to better support talent cultivation in this field. Methods The course at Jinan University was optimized by integrating information technology through online teaching platforms such as XuetangX and Rain Classroom, providing enriched learning resources and fostering guiding, supervision, and motivation. At the same time, multiple teaching modes such as case teaching, interactive Q&A and offline practice are adopted in class to improve students' professional knowledge and practical skills. Finally, with multiple assessment methods, students can combine their own career planning and realize their own interests and strengths so as to achieve individualized development. Results After implementing the blended teaching mode, the number of low - scoring students dropped to zero, while the proportion of high - scoring students increased to 62%. Students’ performance improved significantly compared with pre-reform outcomes, demonstrating the substantial advantages of the blended teaching approach. Conclusion Through the "online and offline hybrid" teaching method, the content and teaching objectives of biotechnical drug course are optimized, which provides a reference for the future construction and teaching reform of this course.